Evaluation the Susceptibility of Metformin Drug and Pollen grain of Typha domengensis (Typha) on Prevention of Damage in octa4 gene Which Induced by Mitomycin c in Some Organs in Male Albino Rats

Abstract

Background: Living organisms are permanently exposed to internal and external factors that damage DNA. This damage, if not repaired, can lead to the mutation which cause verius diseases and cellular death. Therefore,the use of many plants or their products has been adopted as aprotective material against genetic toxicityof certain drugs.

Objective:Examined the susceptibility of both metformin and pollen grain solution to the prevention of damage in octa4 gene and mitomycin C, In order to be adopted as materials available and cheap to prevent damage to genetic material and the result of the use of human drugs.                                                                                                                                      Patients and Methods: was used 25 white males  rats for 4 weeks obtained from the College of Veterinary Medicine / University of Baghdad and ages ranging from (10-12) weeks. The  rats were divided into 5 groups. the control group was drip with distilled water as well as injected The second  group was treated with mitomycin (0.001ml) was injected subcutaneously weekly for 30 days.while the third group was orally injected with metformin with a concentration of 500 mg / kg with mitomycin  , while the fourth  group was treated with mitomycin and water solution for pollen at 0.1 g / mL per animal. while the fifth group included rats treated with mitomycin with metformin and water solution for pollen. The animals were anesthetized and the kidney and liver were removed. The DNA extraction process was investigated and the presence of the octa4 gene was investigated using a special technique and PCR technique.The tissues like liver, kidney and testies were collected for study the expression of Oct 4 gene.

Results: The C. papyrus pollon extract along with metformin is effective against mitomicine C induced animals. Oct 4 it has been protected from mitomycin damage  in both the kidney and liver tissue compared to the testis of the animals treated with C. papyrus pollon extract and metformin. C. papyrus pollen grain producing an effective and safe product, and further research is required in addition to the aggregate value to the native plants.

Conclusion: In the present study, C. papyrus pollon extract along with metformin is effective against mitomicine C induced animals. Oct 4 expression was significantly higher in the kidney and liver tissue of the animals treated with C. papyrus pollon extract and metformin. The result of the present study clearly indicates that C. papyrus reduces blood glucose levels, producing an effective and safe product, and further research is required in addition to the aggregate value to the native plants.