The Inhibition Effect of Paliurus Spina-Christi Methanolic Extract on Trichophyton Mentagrophytes Growth and Tri M4 Gene

Abstract

Back ground: Paliurus spina-christi  methanolic extract  is  rich of phenolic compounds  as well as other biological active compounds (sugars ,esters, fatty acids and alkaloids) .

Aim:To asses that Paliurus spina-christi a good anti dermatophytic  activity (Trichophyton mentagrophytes) .

Materials and methods: five different concentrations of methanolic  extract of P. spina-christi against T. mentagrophytes followed by the RNA extraction  of Ttichophyton mentagrophytes grown in media containing P. spina-christi methanolic extract   followed by complementary DNA synthesis to examine the expression of Tri m4 gene .

Results: Statistical analysis revealed that at concentrations(25 and 50)µg/ml the differences between means of  the growth diameters are non-significant (P≥ 0.05)  as compared with the control  while at concentrations (75,100,125)µg/ml the effect of methanolic extract on diameter growth  was statistically significant (P≤0.05). Maximum growth inhibition was at 125µg/ml while minimum inhibition was at concentration 25 µg/ml  .The percentage of inhibition was statistically significant especially at concentration 125 µg/ml it  recorded 82%. The expression of Tri m4 gene was identified in presence of extract .

 Conclusion :Methanolic extract of P.   spina-christi didn’t affect the expression of Tri m4 gene.