Effect alkaloid Narciclasine Extraction of Zephyranthes candida On Microtubulues of HepG2 Hepatic Cell Line

Abstract

Background: Alkaloid  narciclasine extraction of Zephyranthes candida are microtubular toxins of chemically similar nature that disrupt microtubule function by binding to a site on β-tubulin and suppressing microtubule dynamics.effect in hepatic cell line.

Objective: To evaluate biological activity of alkaloid  narciclasine extraction of Zephyranthes candida on microtubule HepG2 cell line by used GF tubulin with alcoholic extraction.                                                                                   

Patients and Methods: The experiments work in China (Wuhan) 2016 need about 6 months , we used  TLC technology to extraction alkaloids narciclasine from by used different concentration 20,40,80,100,200,400 μg/ml for one hours to microtubule cell line of HepG2 cell line and used high concentration 1000,2000 μg/ml to investigate the action of alkaloids  narcilacsine onto the network of microtubule to one hours .

Results: Increased the number of the cell effect by extraction in microtubule by used GF tubulin inside the cell by alkaloid or alcoholic extraction see the results by immunofluorescence microscope, the microtubule is effect by high concentration of extraction see apoptosis and thinned down, and individual fibres have a wavelike shape.   Anther experimental work was conducted to determine the biological activity of alkaloids Narcilasine on microtubule  in concentration 20 μg/ml in (12,10,8,6) hours incubation and 400 μg/ml for 10 min  to study   cell line and the recovery of its disruption.Cells were treated with alkaloids at various concentrations from 20 μg/ml to 400 μg/ml for 60 min microtubules were  recovered and  network is nearly restored.the results show the microtubulesis back normal after reduce extraction alcoholic and alkaloids in 12 hours by used  indirect immunofluorescence.

Conclusion: The cells showed changes in the arrangement of microtubules even at the 80 μg/ml concentration of cytostatics after 60-min exposition. Its damage increased with increasing concentration of cytostatics.