Study of Antibacterial Activities of Seeds Extract of Fenugreek (Trigonella foenum-graecum)

Abstract

Background: The antibacterial activity of seeds  methanol extract and hot aqueous extract  of fenugreek (Trigonella foenum-graecum) showed the  presence of  activity  against four species of bacteria and the methanol extract have higher activity than other extract.

Objective: To examine the antimicrobial activities of the crude methanolic extract  and hot aqueous extract of seeds of Trigonella foenum-graecum.

Patients and Methods: We prepared three different concentrations  for all extracts ( 75% ,50%, 25%,   v/v ). The study detected antibacterial activity  by using well diffusion in agar technique .The study  applied on the Four species of bacteria   includes :Staphylococcus aureus , Bacillus subtilus, Pseudomonas aeruginosa and Escherichia coli.  The study continued from September  2016 to January 2017 in the Laboratory of Biotechnology  in College of Science - University of Diyala.

Results: The study showed highest antibacterial activity of  methanolic extract of seeds of Trigonella foenum –graecum   that attained highest zone of inhibition (30 mm) against  P. aeruginosa  when using (75%) concentration,While attained lowest zone of inhibition (10 mm) against  E.coli when using (25%) concentration. Whereas the hot aqueous  extract of  seeds of Trigonella foenum –graecum    that attained highest zone of inhibition (24 mm) against P. aeruginosa when using (75%) concentration, while  don't give any effect against bacterial spp. when using (25%)concentration. 

Conclusion: Antibacterial activity of methanol extract of seeds of Trigonella foenum-graecum   was higher than the hot aqueous extract also higher than  commercial antibiotic (Amikacin).