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Jasim Hadi AL-Anbagi Nazar Jabbar AL-Khafaji

Abstract

Background :
During the present ways of handling of meat , processing and transport, starting from slaughters r houses till reach the consumers, it is expected that great amount and type of zoonotic bacteria may contaminate and pollute the meat and meat products in Diyala province \ Iraq. Accordingly it is investigated in the present research to identify and differentiate the commonest contaminant bacteria that may isolate from samples were collected, from meat and meat products of slaughtered sheep's and cow, and poultry’s meat, and swabs from workers and their equipment that are used in butchery shops, during the period from August, 2019 to April, 2020.
Objectives :
Isolate and identify and differentiate the commonest zoonotic or contaminant bacteria that may isolate from meat and meat products of slaughtered sheep's and cow, and poultry’s meat, and workers and their equipment that are used in butchery shops in Diyala province, and follow the antibiotics that may be effective against such isolates .
Materials and methods:
A total 251 samples were collected, 35 sheep's and cow's meat and meat products, 41 samples poultry’s meat, and 175 swabs from workers and their equipment that are used in butchery shops, in the period from August, 2019 to April, 2020. The samples submitted to laboratory investigation to isolate and identified, the contaminated bacteria, according to their cultural morphology and biochemical properties. Count the total viable bacteria. In addition to study the sensitivity of these bacteria to sixteen commonly used antibiotics. Moreover, the pathogenicity of Klebsiella pneumoniae, which was one of the common isolates in current study, was studied in rabbits .
Results :
The results revealed that Klebsiella spp., Pseudomonas spp., Staphylococcus spp., E. coli, Salmonella spp., Listeria, Enterobacter, Citrobacter, Proteus spp., Yersinia and Shigella were common isolates and the best antibiotics to which nearly all the tested isolates were sensitive, Norfloxacin (Nor 10) and Gentamycin (Cn10) except Listeria; while to Clindamycin (Da10);
Cloxacillin (Cx10); Metromidazole (Met 30); Rifampin (RA5); Amoxicillin (Ax10); Piperacillin (Prl100) all tested isolates were resistant
Conclusion:
The present study searched to identify and differentiate the commonest contaminant bacteria that may isolate from samples were collected, from meat and meat products of slaughtered sheep’s and cow, and poultry’s, meat, and swabs from workers and their equipment that are used in butchery shops. The results revealed that Klebsiella spp., Pseudomonas spp., Staphylococcus spp., E. coli, Salmonella spp., Listeria, Enterobacter, Citrobacter, Proteus spp., Yersinia and Shigella were common isolates and the best antibiotics to which nearly all the tested isolates were sensitive, Norfloxacin (Nor 10) and Gentamycin (Cn10) except Listeria; while to Clindamycin (Da10); Cloxacillin (Cx10); Metronidazole (Met 30); Rifampin (RA5); Amoxicillin (Ax10); Piperacillin (Prl100) all tested isolates were resistant.

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