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Ahmed Hussein AL-Tamimi Nazar Jabbar AL-Khafaji

Abstract

Background: Enterobacteriaceae is a large family of Gram-negative bacteria.
Objectives: Isolate and Identified Enterobacteriaceae of zoonotic importance from poultry 's meat.
Materials and methods: The level of contamination of frozen imported meat with that of local fresh poultry's meat, 70 meat samples from each type of frozen and fresh meat (represented Neck, Wing, Breast and Thigh) were collected from retail shops and slaughterhouse in Diyala, from August 2020 to February 2021. Samples were collected aseptically in clean polyethylene bag and transported to the laboratory in an icebox and submitted to routine procedures of isolation, identification, specification, in dependence on cultural, biochemical characteristics of colonies. Level of contamination in addition to the sensitivities of isolates to 12 antibiotics.
Result: The results revealed that 31 samples out of 140 (22.1%) were free from Enterobactericeae bacterial contamination; while 109 (77.9%) were contaminated.
Conclusion: From frozen meat, the highest isolates were E. coli 27/79(34.2%). While in Fresh meat, the highest one was Klebsiella spp. 30/78(38.5%). The highest viable bacterial counts from frozen meat, from neck log 10(5.50 ±0.01). While from fresh meat, from breast, log 10 (4.85± 0.01). Coliform count from frozen meat, neck log10 (5.46± 0.03); wing (5.00 ±0.04); thigh (4.99 ±0.10) and breast (4.79± 0.08). While from fresh meat, the highest was from thigh log10 (4.42± 0.01); breast (4.41 ±0.07); wing (4.36± 0.02); and neck 3 (4.32± 0.08). The best antibiotics to which all isolates were sensitive were Amikacin (AK30μg); Chloramphenicol (C30 μg); Ceftriaxone (CRO 30 μg) Cefepime (FEP 30 μg). At the same time all isolates were resistant to Amoxicillin (AML25 μg) and Ampicillin (AMP 10 μg).

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