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K. pneumoniae Gram negative bacteria, with large capsule, fermented lactose of Enterobacteriaceae family, it is part of the normal flora in the intestines, skin and mouth, but can cause several opportunistic nosocomial and community acquired infection in human, also can cause different infection in animal. Objectives: Isolation and Identification of K. pneumoniae from human and domesticated animals. Materials and methods:. A total of 182 specimens from human include; blood, urine, stool, sputum specimens, wound and burn swabs collected from hospitalized and out patients of both sexes, in age one to 65 years. Furthermore, 111 different specimens collected from different animal species including goats, sheep, chicken and cows. Animal specimens include stool, milk, and urine. K. pneumoniae diagnosis done by use selective media for gram negative bacteria of MacConkey and differential culture media of Blood, , EMB (eosin methylene blue), and Urease agar that incubated at 370C for 24 hours. Macroscopically, characteristics for colony, Gram’s stain, Vitek 2 techniques were used in this study. Results: A total of 41 K. pneumoniae isolates were recovered ; (37/182 ) 20.3% from human and (4/111) 3.6% from animal specimens. human isolates were mostly from inpatients. Urine and stool were the most human specimens harboring K. pneumoniae. From the animals side, chicken showed highest isolation rate and stool, milk were the most animal specimens harboring K. pneumoniae. Statistical analysis was done on data accumulated throughout the study analysis performed using SPSS (version25), Chi square, T- test. P values that equal or less than 0.05 were considered significant.