Diyala Journal of Medicine <p style="text-align: justify;">Diyala Journal of Medicine (DJM) was established according to agreement of Ministry of Higher Education and Scientific Research-foundation of as per authorization letter Number 12c/2433 in 19th August 2010. Fellow up, Started to receiving different manuscripts in all fields of medicine such as clinical, basic, dentist, pharmacology and scientific original articles. Diyala Journal of Medicine usually Deal with articles closely related with clinical problem more common in Iraq and Middle East. Also considered is a periodic peer-reviewed journal published biannually by the College of Medicine in Diyala University. However, review articles of high standards will be considered Writing in English languages with Arabic abstract.</p> <p><span style="font-size: 20px; font-family: Times New Roman;"><span style="font-size: 20px; color: #17365d;">&nbsp;</span></span></p> en-US <p>All Rights Reserved.</p> (Salih M. Salman) (Ihsan Ali Hassan) Thu, 16 Aug 2018 00:00:00 +0000 OJS 60 The Cytotoxic Activity Chara elegans on Growth of same Cell Lines <p><strong>Background:</strong>Cellular and molecular studies have shown algae derived ingredients to be potent naturally occurring anticancer compounds and have been suggested to prevent carcinogenesis.Fucoxanthin is a marine carotenoid found in green algae and is documented to display remarkable anticancer activity. Fucoxanthin suppressed the growth of LNCap prostate cancer cells in a concentration-dependent manner along with the induction G(1) cell cycle arrest, but not apoptosis.</p> <p><strong>Objective:</strong> In accordance with same concept, diphlorethohydroxycarmalol (DPHC), isolated from the brown algae Ishige okamurae, notably reduced the level of radiation-induced intracellular ROS and protected cells from undergoing cell death in cultured Chinese hamster lung fibroblast (V79-4) cells.</p> <p><strong>Patients and Methods:</strong> Used leaf&nbsp; extraction from alge chara elegans aqueous extract and crude alcohol. Two types of cell lines, Hela cell line cervical cancer and normal cell embryo rat cell line REF(, were used in this study. They were obtained kindly from Iraqi Center for cancer and medical genetic research (ICCMGR) .The cell line were grown on uncoated coverslips in a Dulbecco’s Minimal Essential Medium (DMEM) with 10 fetal bovine serum (PAA), 2 µM glutamine (PAA), 100 µ/ml penicillin, and 100 μg/ml&nbsp; streptomycin.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <strong>Results</strong>: The results showed this alge contains most of&nbsp; the chemical compounds such as Alkaloids, Saponins, Glycosides, Flavonoids, Steroids and Terpenes.The cytotoxicity of the aqueous extract and crude alcohol extract was investigated&nbsp; on the cancer cells line, hela&nbsp; and normal cell line Ref. Toxic effect for both extracts was indicated by rate of proliferation inhibition . The alcohol extract showed the inhibition of Hela&nbsp; cell line at percentage 20.35%-72.66% more than the aqueous extract (18.68%-48.26%) at concentrations: 62.5-2000 µg/ml. Both extracts (alcoholic&nbsp; extract and aqueous extract) showed almost&nbsp; the same effect at the concentration 8000µg/ml. At low concentrations of both extracts no inhibition effect was observed&nbsp; on normal cells line Ref. The alcohol extract showed the inhibition of Ref&nbsp; cell line at percentage 67.61% more than the aqueous extract (66.66%) at concentrations 8000µg/ml. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<strong>Conclusion</strong>: The conclusion was made that the C. elegans is a promising alge in treatment of cancer through its inhibition&nbsp; of the proliferation of cancer cells., the results demonstrated that&nbsp; extraction from leaf&nbsp; chara elegans aqueous extract and crude alcohol is inhibition Hela cell line&nbsp; cell, and lesser inhibition extend against Ref cell line.</p> Ibrahim Hade Mohammed, Anfal Kadhim Abed, Anfal Kadhim Abed, Qutaiba Hafedth Nsaif, Zena Taha Abud hafez ##submission.copyrightStatement## Thu, 16 Aug 2018 00:00:00 +0000 Isolation and Purification of metalloprotease produced by Aeromonas hydrophila <p><strong>Background:</strong> The bacteria <em>Aeromonas hydrophila</em> was isolated from clinical specimens responsible of causing diarrhea in children and adults.</p> <p><strong>Objective:</strong>To purify metalloprotease enzyme produced by <em>Aeromonas hydrophila</em>.</p> <p><strong>Patients and Methods:</strong> This study involved 150 stool samples collected from patients suffering from diarrhea.</p> <p><strong>Results</strong>: Eight isolate of <em>Aeromonas hydrophila</em> was detected and metalloprotease was purified with 11.06 U/ml of enzyme activity.</p> <p><strong>Conclusion</strong>: The bacteria have ability to produce metalloprotease and enzyme was fully purified in three steps of purification.</p> Muhannad Moeen Abdallah, Tahreer Hadi Saleh ##submission.copyrightStatement## Thu, 16 Aug 2018 00:00:00 +0000 Role of Innate Immune Response Components in the Osteoarthritis <p><strong>Background:</strong>Osteoarthritis is a progressive joint disease is mainly worrying on weight bearing in the body, the joints, particularly the knee and hips. Osteoarthritis as an inflammation in the joints must there is an immune defense in the body against this inflammation. This disease that appears primarily in the elderly characterized by erosion articular cartilage, osteophyte, subchondral bone stiffness, synovitis inflammation, many causes of the disease such as age, sex, and obesity, the location of the joint injury and various other factors.</p> <p><strong>Objective:</strong>To understand the role of innate immune response components in the osteoarthritis, this study was investigated the relation between the innate immune response and osteoarthritis.</p> <p><strong>Patients and Methods:</strong>Fifty osteoarthritis patients and fifty healthy persons were participate in this case-control study. The total WBC count, neutrophil percent, lymphocyte percent, monocyte percent, eosinophil percent, basophile percent, platelet number, level of ESR, Level of CRP, and level of C3 complement were investigate in serum and synovial fluid in the osteoarthritis patients and control healthy persons.</p> <p><strong>Results</strong>: The results were shown significant elevation in the WBC count, platelet number, level of ESR, level of CRP and level of C3 complement in the serum of osteoarthritis patients compared with control group. A significant positive correlation was shown with weight of patient and the severity of osteoarthritis. The elevation level of C3 complement in the synovial fluid was a significant positive correlation with elevation of level of C3, level of ESR, level of CRP, and WBC count in the serum of patients related to the severity of osteoarthritis status.</p> <p><strong>Conclusion</strong>:The results were given an evidence for the crucial role of innate immune response in the defense against osteoarthritis inflammation and any defect in the innate immune component lead to increase the severity of osteoarthritis.</p> Eman Amer Ismail, Ammar N Abood, Walaa Najm Abood, Abass Abood Frahan ##submission.copyrightStatement## Thu, 16 Aug 2018 00:00:00 +0000